Lesson 1
Polymerase Chain Reaction - Way Of Cloning DNA
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Professor Saltzman continues his presentation on DNA technology by discussing control of gene expression using two methods of RNA silencing. Molecular cloning techniques to mass-produce proteins using plasmid, restriction enzymes, ligase, and antibiotic selection in bacteria are discussed. Steps and molecules involved in polymerase chain reaction are also described. Learn how to detect mutations in genomic DNA by gel electrophoresis and Southern blotting. He gives an example of inducing and controlling insulin expression in large animals by cloning into their genome the insulin gene with the lac promoter. Professor Saltzman, W. Mark. BENG 100, Frontiers of Biomedical Engineering, Spring 2008. Yale OpenCourseWare: Biomedical Engineering, http://oyc.yale.edu/biomedical-engineering/beng-100/lecture-4 Accessed 12/9/14. License: Creative Commons BY-NC-SA

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